Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Arsenate suppression of human keratinocyte programming

D J Kachinskas1, Q Qin, M A Phillips

  • 1Department of Environmental Toxicology, University of California, Davis 95616-8588, USA.

Mutation Research
|June 1, 1997
PubMed
Summary

Arsenate exposure prevents keratinocyte differentiation by suppressing key markers. This occurs by altering transcription factors AP1 and AP2, impacting cellular programming.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Polymorphous low-grade neuroepithelial tumor of the young: a molecular pathological study].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2026
Same author

[Protective effect of knock-down the expression of <i>Blimp1</i> gene on early liver injury in CCl<sub>4</sub>-induced mouse model of liver fibrosis].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2025
Same author

Clinical Efficacy of Tobramycin Dexamethasone+Pranoprofen Eye Drops on Endophthalmitis in Cataract Patients Complicated with Diabetes Mellitus after Intraocular Lens Implantation.

Nigerian journal of clinical practice·2025
Same author

Durable benefit from poly(ADP-ribose) polymerase inhibitors in metastatic prostate cancer in routine practice: biomarker associations and implications for optimal clinical next-generation sequencing testing.

ESMO open·2024
Same author

Estimation of the genetic parameters of sheep growth traits based on machine vision acquisition.

Animal : an international journal of animal bioscience·2024
Same author

[Observation of the consistency between intellectualized and manual-based cognitive assessment tools in the outpatient clinic].

Zhonghua yi xue za zhi·2024

Area of Science:

  • Cell biology
  • Dermatology
  • Toxicology

Background:

  • Human keratinocyte line SCC-9 serves as a model for studying arsenate effects on differentiation.
  • Arsenate is known to perturb cellular differentiation processes.

Purpose of the Study:

  • To investigate the molecular mechanisms by which arsenate suppresses keratinocyte differentiation.
  • To determine the role of transcription factors AP1 and AP2 in arsenate-induced suppression of involucrin expression.

Main Methods:

  • Northern blotting to assess mRNA levels of differentiation markers.
  • Promoter analysis to investigate the impact of mutations on transcriptional activity.
  • Treatment of SCC-9 cells with arsenate, vanadate, chromate, and tetradecanoylphorbol acetate.

Related Experiment Videos

Main Results:

  • Arsenate (10 microM) prevented the expression of six suprabasal differentiation markers in SCC-9 cells.
  • Vanadate and chromate exhibited similar suppressive effects on differentiation markers as arsenate.
  • Mutations in AP1 and AP2 response elements significantly reduced involucrin promoter activity, supporting their role in arsenate's effect.

Conclusions:

  • Arsenate suppresses keratinocyte differentiation, including involucrin expression, by modulating the activity of transcription factors AP1 and AP2.
  • These findings suggest a broader impact of arsenate on keratinocyte programming through transcription factor alteration.