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Protein kinase C activity and expression in rabbit left ventricular hypertrophy
K Mohammadi1, P Rouet-Benzineb, M Laplace
1Unité INSERM 400, Faculté de médecine, Créteil, France.
Journal of Molecular and Cellular Cardiology
|June 1, 1997
Summary
Protein Kinase C (PKC) activity and expression decrease in rabbit ventricular overload, differing from rat models. This suggests species-specific responses in myocardial hypertrophy and that hypertrophy can occur without increased PKC.
Area of Science:
- Cardiology
- Molecular Biology
- Biochemistry
Background:
- Protein Kinase C (PKC) is linked to myocardial hypertrophy.
- Previous studies show conflicting PKC activity/expression in rat vs. rabbit hypertrophy models.
Purpose of the Study:
- Investigate species and model differences in PKC activity and expression during rabbit ventricular overload.
- Determine if PKC is essential for hypertrophy induction.
Main Methods:
- Induced mild ventricular overload in rabbits via aortic constriction.
- Measured PKC activity and expression in particulate and cytosolic fractions of left ventricular tissue.
- Utilized immunoblotting for PKC content analysis.
Main Results:
- Rabbit ventricular overload led to decreased particulate PKC activity and content.
- PKC translocation to the particulate fraction was significantly reduced.
- Hypertrophy (increased LV weight/body weight ratio) occurred without increased PKC induction.
Conclusions:
- Demonstrates species-specific differences in PKC response to hypertrophy between rats and rabbits.
- Suggests that myocardial hypertrophy can develop independently of PKC induction.