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Abstract:
Absorption studies of hydrogen bonding association between nucleic acid bases and amino acid side chains show that association constants can be determined from difference absorption spectra in cyclohexane and chloroform. Association constants for the binding of 9-ethyladenine, 1-cyclohexyluracil and 1-cyclohexylcytosine to side chains of serine, threonine, aspartic acid, lysine, cystéine, methionine and tyrosine are reported. Results obtained in chloroform and cyclohexane are compared.