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[Two simple methods for isolation of DNA from various sources using cetavlon]
Biokhimiia (Moscow, Russia)
|October 1, 1977
Summary
Two methods using cetyltrimethylammonium bromide (CTA-Br) efficiently isolate pure DNA from diverse sources. These rapid techniques yield high-quality DNA with approximately 80% recovery.
Area of Science:
- Molecular Biology
- Biochemistry
Background:
- DNA isolation is crucial for molecular biology applications.
- Existing methods can be time-consuming or require specific enzymes.
Purpose of the Study:
- To describe two novel, rapid methods for DNA isolation.
- To utilize cetyltrimethylammonium bromide (CTA-Br) for DNA precipitation.
Main Methods:
- Method 1: DNA precipitation as a CTA-salt after cell lysis, deproteinization with chloroform, and salt concentration adjustment.
- Method 2: DNA purification on a hydroxyapatite column post-cell lysis and debris removal.
Main Results:
- Both methods provide rapid isolation of pure DNA from various sources.
- High DNA recovery (approx. 80%) and high average molecular weight (≥20x10^6) were achieved.
- No need for ribonuclease, pronase, or amylase was demonstrated.
Conclusions:
- CTA-Br offers an effective detergent for DNA isolation.
- These methods are efficient, rapid, and yield high-quality DNA suitable for downstream applications.