Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simplified quantitative cervical mucus penetration test

G N Clarke1

  • 1Division of Pathology, The Royal Women's Hospital, Carlton, Victoria, Australia.

Human Reproduction (Oxford, England)
|June 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prevention of Depression in At-Risk Adolescents: Moderators of Long-term Response.

Prevention science : the official journal of the Society for Prevention Research·2016
Same author

Hyperactivation of capacitated human sperm correlates with the zona pellucida-induced acrosome reaction of zona pellucida-bound sperm.

Human reproduction (Oxford, England)·2007
Same author

Antisperm antibodies and conception.

Seminars in immunopathology·2007
Same author

Tyrosine phosphorylation on capacitated human sperm tail detected by immunofluorescence correlates strongly with sperm-zona pellucida (ZP) binding but not with the ZP-induced acrosome reaction.

Human reproduction (Oxford, England)·2006
Same author

Hyper-osmotic condition enhances protein tyrosine phosphorylation and zona pellucida binding capacity of human sperm.

Human reproduction (Oxford, England)·2005
Same author

Exposure of actin on the surface of the human sperm head during in vitro culture relates to sperm morphology, capacitation and zona binding.

Human reproduction (Oxford, England)·2005
Same journal

Characterizing endometriosis and adenomyosis symptom clusters and their impact on quality of life in the All of Us Research Program.

Human reproduction (Oxford, England)·2026
Same journal

A new regulatory framework: impact of the SoHO Regulation for medically assisted reproduction.

Human reproduction (Oxford, England)·2026
Same journal

Subfertility in relation to gestational weight gain and gestational diabetes: the Norwegian Mother, Father and Child Cohort Study.

Human reproduction (Oxford, England)·2026
Same journal

Pathogenic variants in ACTRT3 lead to male infertility characterized by fertilization defects.

Human reproduction (Oxford, England)·2026
Same journal

A large retrospective study on 1PN embryo transfer supports the need for updated harmonized best practice guidelines.

Human reproduction (Oxford, England)·2026
Same journal

Explainability of artificial intelligence (AI) tools in assisted reproductive technologies (ARTs): promise, clinical reality, and conceptual limits.

Human reproduction (Oxford, England)·2026
See all related articles

A new simplified cervical mucus penetration test shows sperm concentration in mucus mainly depends on progressive sperm count. The motile density ratio may serve as a practical index for sperm penetration efficiency in clinical settings.

Area of Science:

  • Reproductive Biology
  • Sperm Analysis
  • Infertility Diagnostics

Background:

  • Cervical mucus penetration by spermatozoa is a critical factor in natural conception.
  • Accurate assessment of sperm-mucus interaction is essential for diagnosing male infertility.
  • Existing methods for evaluating sperm penetration can be complex and time-consuming.

Purpose of the Study:

  • To develop and evaluate a simplified quantitative cervical mucus penetration test.
  • To identify key semen parameters influencing sperm penetration into cervical mucus.
  • To assess the clinical utility of derived penetration efficiency indices.

Main Methods:

  • A simplified quantitative cervical mucus penetration test was applied to semen samples from 21 prospective donors.

Related Experiment Videos

  • Stepwise multiple regression analysis was used to correlate semen parameters with sperm concentration in mucus.
  • Indices of penetration efficiency, including percentage successful collisions (PSC) and motile density ratio (MDR), were calculated and analyzed.

    • Main Results:

    • Spermatozoa concentration in cervical mucus was primarily correlated with progressive spermatozoa concentration (63% variance explained).
    • Average path velocity of spermatozoa in semen further explained variance in mucus penetration (70% combined).
    • Penetration efficiency indices (PSC, PSE, MDR) showed no significant correlation with semen variables, though models explained 27-31% of variance.

    Conclusions:

    • Spermatozoa concentration in cervical mucus is strongly linked to semen quality, particularly progressive motility and velocity.
    • The motile density ratio (MDR) demonstrates a strong correlation with the percentage of successful collisions (PSC).
    • The MDR may serve as a simplified, clinically useful index for assessing sperm penetration efficiency, warranting further research.