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Related Experiment Videos

A highly temperature-sensitive proton current in mouse bone marrow-derived mast cells

M Kuno1, J Kawawaki, F Nakamura

  • 1Department of Physiology, Osaka City University Medical School, Japan. kunomyk@msic.med.osaka-cu.ac.jp

The Journal of General Physiology
|June 1, 1997
PubMed
Summary

This study reveals a temperature-sensitive proton (H+) conductive pathway in mast cells crucial for regulating intracellular pH. This pathway

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Area of Science:

  • Cellular Physiology
  • Ion Transport Mechanisms
  • Mast Cell Biology

Background:

  • Proton (H+) conductive pathways are implicated in maintaining intracellular pH homeostasis.
  • Mast cells play critical roles in immune responses and inflammation.
  • Understanding ion transport in mast cells is vital for cellular function.

Purpose of the Study:

  • To characterize temperature-sensitive whole-cell currents in mouse bone marrow-derived mast cells (BMMC).
  • To investigate the role of proton (H+) conductive pathways in BMMC intracellular pH regulation.
  • To elucidate the properties and regulation of a novel H+ conductance.

Main Methods:

  • Whole-cell patch-clamp electrophysiology to measure temperature-sensitive currents in BMMC.
  • Manipulation of intracellular and extracellular pH to assess current characteristics.

Related Experiment Videos

  • Fluorescent dye (BCECF) measurements to monitor intracellular pH changes.
  • Pharmacological inhibition using Zn2+ and bafilomycin A1.

    • Main Results:

    • A voltage-dependent outward conductance, activated by heating (Q10 ≈ 10), was identified in BMMC.
    • The current's reversal potential depended on the pH gradient, indicating H+ as the charge carrier.
    • Intracellular pH regulation during acid-load was modulated by temperature, Zn2+, and extracellular K+.

    Conclusions:

    • A temperature-sensitive H+ conductive pathway significantly contributes to intracellular pH homeostasis in BMMC.
    • The high activation energy of this pathway may enhance H+ conductance.
    • This finding provides new insights into the mechanisms of pH regulation in mast cells.