Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative trait loci controlling halothane sensitivity in Caenorhabditis elegans

B van Swinderen1, D R Shook, R H Ebert

  • 1Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Proceedings of the National Academy of Sciences of the United States of America
|July 22, 1997
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AGRICULTURAL BEST MANAGEMENT PRACTICE SENSITIVITY TO CHANGING AIR TEMPERATURE AND PRECIPITATION.

Transactions of the ASABE·2021
Same author

Corrigendum to "Bacterial mutagenicity screening in the pharmaceutical industry" [Mutat. Res. 752(2) (2013) 99-118].

Mutation research·2020
Same author

The effects of downscaling method on the variability of simulated watershed response to climate change in five U.S. basins.

Earth interactions·2018
Same author

Nicotinamide mononucleotide adenylyltransferase promotes hypoxic survival by activating the mitochondrial unfolded protein response.

Cell death & disease·2016
Same author

Rapid memory T-cell reconstitution recapitulating CD45RA-depleted haploidentical transplant graft content in patients with hematologic malignancies.

Bone marrow transplantation·2015
Same author

Application of computational models to estimate organ radiation dose in rainbow trout from uptake of molybdenum-99 with comparison to iodine-131.

Journal of environmental radioactivity·2015

Genetic analysis reveals significant natural variation in volatile anesthetic (VA) sensitivity in C. elegans. A major genetic locus on chromosome V explains over 40% of this variation in response to halothane.

Area of Science:

  • Neuroscience
  • Genetics
  • Pharmacology

Background:

  • Volatile anesthetics (VA) disrupt nervous system function, but the genetic basis of sensitivity variation is poorly understood.
  • Previous mutagenesis efforts have yielded limited success in identifying resistant strains.
  • A quantitative genetic approach is needed to explore natural variation in VA sensitivity.

Purpose of the Study:

  • To investigate the natural variation in sensitivity to the volatile anesthetic halothane in Caenorhabditis elegans.
  • To identify genetic loci influencing halothane sensitivity in behavior.
  • To determine if identified loci affect basal behavior or anesthetic metabolism.

Main Methods:

  • Utilized recombinant-inbred strains of C. elegans derived from distinct lineages.

Related Experiment Videos

  • Assessed halothane sensitivity (EC50) for coordinated movement and male mating behavior.
  • Employed quantitative trait loci (QTL) mapping to identify genetic determinants.

    • Main Results:

    • Demonstrated significant heritability and wide variation (13- and 4-fold ranges) in halothane sensitivity for movement and mating.
    • Identified a major locus on chromosome V explaining >40% of phenotypic variance for both traits.
    • Discovered five weaker, interacting loci contributing to the remaining variance.

    Conclusions:

    • Natural genetic variation significantly impacts volatile anesthetic sensitivity in C. elegans.
    • A major locus on chromosome V and several minor loci control halothane sensitivity.
    • Identified QTL likely represent direct targets or downstream effectors of halothane in the nervous system, not basal behavior or metabolism differences.