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Sorbitol accumulation and transmembrane efflux in osmotically stressed JS1 schwannoma cells
A P Mizisin1, L Li, N A Calcutt
1Department of Pathology, School of Medicine, University of California, San Diego, La Jolla 92093-0612, USA. amizisin@ucsd.edu
Neuroscience Letters
|June 20, 1997
Abstract:
Sorbitol accumulation and transmembrane efflux in JS1 schwannoma cells were examined during osmotic stress in the presence of a sorbitol dehydrogenase (SD) inhibitor and following return to iso-osmotic conditions. SD inhibition promoted sorbitol accumulation under hyperglycemic and/or hyperosmotic conditions, and sorbitol efflux during iso-osmotic incubation was prevented by cooling to 4 degrees C or by quinidine. It appears that sorbitol levels in JS1 cells are dependent on SD activity and that sorbitol is rapidly removed upon restoring iso-osmotic conditions.