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Type-4 pilus-structure: outside to inside and top to bottom--a minireview
1Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Gene
|June 11, 1997
Summary
We developed a computational model of the Neisseria gonorrhoeae pilus fiber, revealing a three-layered structure. This model aids understanding of type-4 pilus assembly and function.
Area of Science:
- Microbiology
- Structural Biology
- Biophysics
Background:
- The Neisseria gonorrhoeae pilus is a crucial virulence factor.
- Previous studies established the X-ray crystal structure of pilin and biophysical data.
Purpose of the Study:
- To present an updated computational model of the N. gonorrhoeae pilus fiber.
- To identify exposed regions of pilin within the assembled fiber using antibody mapping.
Main Methods:
- Computational modeling based on X-ray crystal structure of pilin.
- Biophysical and genetic data integration.
- Anti-peptide antibody mapping to identify exposed epitopes.
Main Results:
- The N. gonorrhoeae pilus fiber exhibits a three-layered structure.
- Coiled conserved alpha helices are surrounded by beta-sheet.
- The hypervariable region is the most exposed portion of the pilus.
Conclusions:
- The developed pilus model is representative of type-4 pili.
- The model has implications for understanding type-4 pilus assembly and function.
- Insights into pilus interactions with other proteins and cell membranes are provided.