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Published on: November 16, 2012
A ribosomal RNA operon from Pseudomonas stutzeri Zobell
1Institute of Marine and Coastal Sciences, Rutgers University, New Brunswick, NJ 08903-0231, USA. kerkhof@ahab.rutgers.edu
Abstract:
A ribosomal RNA operon from the marine bacterium, Pseudomonas stutzeri Zobell, was cloned and characterized by Southern hybridization and sequence analysis. The 16S rRNA, 23S rRNA, 5S rRNA and 2 tRNA genes (alanine and isoleucine) were identified by homology with sequences in GenBank. The rRNA gene exhibited typical eubacterial organization (16S-tRNAs-23S-5S). A putative ribosomal promoter and anti-terminator regions were also identified and described. Significant differences in spacing of the anti-terminator regulatory elements were observed between P. stutzeri Zobell and Escherichia coli.
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