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Related Experiment Videos

Polymorphism and Distribution of Apo(a)

A Abe1, M Seishima, S Maeda

  • 1Department of Laboratory Medicine, Gifu University School of Medicine, Japan.

Journal of Atherosclerosis and Thrombosis
|January 1, 1995
PubMed
Summary

Researchers identified several apolipoprotein(a) [apo(a)] isoforms in healthy Japanese individuals. They found an inverse relationship between apo(a) molecular weight and plasma lipoprotein(a) [Lp(a)] levels, suggesting Lp(a) binds to triglyceride-rich lipoproteins.

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Area of Science:

  • Biochemistry
  • Genetics
  • Cardiovascular Science

Background:

  • Lipoprotein(a) [Lp(a)] is a lipoprotein particle that plays a role in cardiovascular disease.
  • Apolipoprotein(a) [apo(a)] is the primary apolipoprotein found in Lp(a).
  • The genetic control and physiological function of Lp(a) and apo(a) are not fully understood.

Purpose of the Study:

  • To investigate the different isoforms of apo(a) in a healthy Japanese population.
  • To determine the relationship between apo(a) isoforms and plasma Lp(a) concentrations.
  • To explore the interaction of Lp(a) with other lipoproteins, particularly after a fat load.

Main Methods:

  • Analysis of 470 healthy Japanese individuals.
  • Utilized 4% SDS-PAGE and immunoblotting techniques to identify apo(a) isoforms.

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  • Measured plasma Lp(a) concentrations and analyzed lipoprotein fractions after oral fat loading.

    • Main Results:

    • Identified several apo(a) isoforms, including Lp(a)F, Lp(a)B, Lp(a)S1-S4, and null alleles.
    • Demonstrated a significant inverse correlation between the apparent molecular weight of apo(a) isoforms and plasma Lp(a) levels.
    • Observed an increase in Lp(a) in the d < 1.006 fraction (triglyceride-rich lipoproteins) 2-4 hours post-oral fat load.
    • Showed that Lp(a) has a high affinity for triglyceride-rich lipoproteins (TRLs).
    • Confirmed that lipid-free apo(a) in serum, with a molecular mass of approximately 200 kDa, does not contain apo B-100.
    • Found no significant difference in free apo(a) levels between normal subjects and patients with coronary artery disease (CAD).

    Conclusions:

    • The diversity of apo(a) isoforms influences plasma Lp(a) concentrations.
    • Triglyceride-rich lipoproteins may serve as a carrier for intact Lp(a) in circulation.
    • The findings contribute to understanding the metabolism and potential role of Lp(a) in cardiovascular health and disease.