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Esterases A5-B5 in organophosphate-resistant Culex pipiens from Italy
C Severini1, R Romi, M Marinucci
1Department of Parasitology, Istituto Superiore di Sanità, Rome, Italy.
Medical and Veterinary Entomology
|April 1, 1997
Abstract:
Culex pipiens mosquitos from Lignano city, Udine province, northeast Italy, were found to carry over-produced non-specific esterases A1, A2-B2 and A4-B4 or A5-B5, detected by starch gel electrophoresis, giving multiple resistance to organophosphorus insecticides. In order to differentiate between A4-B4 and A5-B5 esterases, the latter known only from Cyprus whereas the former is widespread in Italy and elsewhere, restriction fragment length polymorphism (RFLP) analysis was performed at the esterase B locus. Both B4 and B5 haplotypes were found. This is the first record of A5-B5 esterase-mediated resistance in continental Europe.