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Opsonic effect of equine plasma from different donors
G Gröndahl1, A Johannisson, M Jensen-Waern
1Department of Medicine and Surgery, Swedish University of Agricultural Sciences, Uppsala, Sweden. gittan.grondahl@kirmed.slu.se
Veterinary Microbiology
|June 16, 1997
Summary
Equine plasma donor choice significantly impacts neutrophil phagocytosis in both foals and adult horses. Individual variations in plasma opsonic activity exist, even with similar immunoglobulin G levels.
Area of Science:
- Veterinary Immunology
- Equine Health
Background:
- Phagocytosis is a critical immune function in neutrophils.
- Equine plasma's role in enhancing neutrophil phagocytosis requires further investigation.
Purpose of the Study:
- To assess the impact of different equine plasma donors on neutrophil phagocytic capacity.
- To compare the opsonic activity of plasma from various donors in both foals and adult horses.
Main Methods:
- Neutrophils from foals and adult horses were incubated with fluorescent yeast cells.
- Opsonization was performed using plasma from three individual donors or pooled serum.
- Flow cytometry was used to quantify phagocytic neutrophils and ingested yeast cells.
Main Results:
- Plasma from donor GN significantly enhanced neutrophil phagocytosis compared to other donors and pooled serum (p < 0.05).
- Heat-inactivated plasma markedly reduced phagocytosis, indicating the importance of complement or heat-labile factors.
- Autologous foal sera generally showed lower opsonic capacity than adult donor plasma.
Conclusions:
- Significant individual variation exists in the opsonic activity of equine plasma donors.
- Plasma donor selection can influence neutrophil function in horses, regardless of age.
- Understanding donor variability is crucial for optimizing plasma-based therapies in equine medicine.