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Related Experiment Videos

Polymorphic microsatellite DNA markers in the rabbit (Oryctolagus cuniculus)

W A van Haeringen1, M den Bieman, L F van Zutphen

  • 1Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Graduate School of Animal Health, Utrecht University, The Netherlands.

Journal of Experimental Animal Science
|January 1, 1996
PubMed
Summary

Rabbit microsatellites from the EMBL database show high polymorphism. These genetic markers are valuable for diverse rabbit breeds, aiding in genetic studies and marker-assisted selection.

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Area of Science:

  • Animal Genetics
  • Molecular Biology
  • Genomics

Background:

  • Microsatellites are repetitive DNA sequences widely used as genetic markers.
  • The EMBL nucleotide database is a valuable resource for identifying potential genetic markers.
  • Understanding genetic diversity in rabbit breeds is crucial for conservation and breeding programs.

Purpose of the Study:

  • To evaluate the polymorphic potential of rabbit nuclear gene microsatellites identified from the EMBL database.
  • To assess the utility of these microsatellites as genetic markers in different rabbit breeds.

Main Methods:

  • Searched the EMBL nucleotide database to identify rabbit nuclear gene microsatellites.
  • Analyzed 13 microsatellite sequences using Polymerase Chain Reaction (PCR) to assess polymorphism.

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  • Examined amplified fragments in rabbits from various breeds.

    • Main Results:

    • Identified 157 rabbit nuclear gene microsatellites.
    • The 13 analyzed microsatellites yielded polymorphic products with an average of four alleles per sequence (range: 2-11).
    • A positive correlation was observed between repeat unit length and the number of alleles detected.

    Conclusions:

    • Rabbit microsatellites from the EMBL database exhibit sufficient polymorphism for genetic studies.
    • These microsatellites are suitable as Type 1 markers for rabbit genetic research.
    • The findings support the use of bioinformatic approaches for identifying useful genetic markers.