Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure of sinuses in the human lymph node

P G Forkert, J A Thliveris, F D Bertalanffy

    Cell and Tissue Research
    |September 14, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Acridine Orange Fluorescence in Cytology.

    Canadian Medical Association journal·2010
    Same author

    The effect of elevated dietary cholesterol on pulmonary surfactant function in adolescent mice.

    Pediatric pulmonology·2008
    Same author

    DNA fragmentation in developing lung fibroblasts exposed to Stachybotrys chartarum (atra) toxins.

    Pediatric pulmonology·2007
    Same author

    Structural parameters of the vastus medialis muscle.

    Clinical anatomy (New York, N.Y.)·2005
    Same author

    Stachybotrys chartarum alters surfactant-related phospholipid synthesis and CTP:cholinephosphate cytidylyltransferase activity in isolated fetal rat type II cells.

    Toxicological sciences : an official journal of the Society of Toxicology·2004
    Same author

    Dietary induction of NQO1 increases the antitumour activity of mitomycin C in human colon tumours in vivo.

    British journal of cancer·2004

    This study used a novel casting technique to visualize human lymph node microcirculation. Findings reveal gaps in sinus walls, facilitating cell movement between the sinus lumen and lymphoid parenchyma.

    Area of Science:

    • Immunology
    • Anatomy
    • Microcirculation

    Background:

    • The human lymph node's intricate microcirculation is crucial for immune responses.
    • Understanding lymphatic sinus structure is key to comprehending immune cell trafficking.

    Purpose of the Study:

    • To visualize the three-dimensional lymphatic microcirculation within the human lymph node using a novel casting technique.
    • To investigate the structural integrity of lymphatic sinuses and their role in cell transit.

    Main Methods:

    • A specialized casting compound was used to fill and visualize the lymphatic microcirculation.
    • Three-dimensional casts of the lymph node's internal structures were created.
    • Electron microscopy was employed to examine the endothelial lining of the lymphatic sinuses.

    Related Experiment Videos

    Main Results:

    • Casting revealed well-defined channels within the lymph node, converging into efferent lymphatics.
    • The outer sinus walls and trabecular sinus sides exhibited intact endothelium.
    • Gaps were observed in the inner marginal sinus wall and parenchymal wall of trabecular sinuses, particularly in the medulla.

    Conclusions:

    • Lymphatic sinus walls adjacent to connective tissue act as barriers, while those near lymphoid cells have gaps.
    • These structural features facilitate cell movement between the sinus lumen and the lymphoid parenchyma.
    • The findings provide new insights into immune cell trafficking within human lymph nodes.