Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The 26S proteasome: subunits and functions

K Tanaka1, C Tsurumi

  • 1The Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Japan.

Molecular Biology Reports
|March 1, 1997
PubMed
Summary

The 26S proteasome is a large eukaryotic complex that degrades proteins. Its structure includes a catalytic core and regulatory modules, with ongoing research into its subunits.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vivo evaluation of a novel albumin-binding prodrug of doxorubicin in an orthotopic mouse model of prostate cancer (LNCaP).

Prostate cancer and prostatic diseases·2010
Same author

The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor.

The EMBO journal·1998
Same author

cDNA cloning of bovine thrombospondin 1 and its expression in odontoblasts and predentin.

Biochimica et biophysica acta·1998
Same author

Proteasomes and antigen processing.

Advances in immunology·1997
Same author

[Proteolysis and cell cycle regulation].

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme·1996
Same author

cDNA cloning and functional analysis of the p97 subunit of the 26S proteasome, a polypeptide identical to the type-1 tumor-necrosis-factor-receptor-associated protein-2/55.11.

European journal of biochemistry·1996

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • The 26S proteasome is a large, ATP-dependent protease complex crucial for eukaryotic protein degradation.
  • It comprises a central 20S catalytic core and two V-shaped terminal regulatory modules.

Purpose of the Study:

  • To describe the structural composition of the 26S proteasome.
  • To highlight the ongoing research into its regulatory subunits.

Main Methods:

  • Recombinant DNA techniques were used to determine the primary structures of 20S proteasome subunits.
  • Structural analyses of 26S proteasome regulatory subunits are currently in progress.

Main Results:

  • The 26S proteasome has a molecular mass of approximately 2000 kDa.
  • Regulatory subunits are divided into ATPases (at least 6) and non-ATPase subunits (approximately 15).
  • Homologous polypeptides encoding ATPases are conserved across evolution.

Conclusions:

  • Understanding the 26S proteasome's subunit structure is vital for comprehending its regulatory roles.
  • Further structural analysis of regulatory subunits is needed to fully elucidate the complex's function.

Related Experiment Videos