Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The 26S proteasome: a dynamic structure

M Seeger1, K Ferrell, W Dubiel

  • 1Institute of Biochemistry, Medical Faculty (Charité), Humboldt University, Berlin, Germany.

Molecular Biology Reports
|March 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Geographical variability of bacterial communities of cryoconite holes of Andean glaciers.

Scientific reports·2023
Same author

Bioremoval of copper by filamentous fungi isolated from contaminated soils of Puchuncaví-Ventanas Central Chile.

Environmental geochemistry and health·2023
Same author

First Report of <i>Diaporthe ambigua</i> Associated with Dead Arm Disease on Grapevine in Chile.

Plant disease·2021
Same author

Complete genome sequence of the marine Rhodococcus sp. H-CA8f isolated from Comau fjord in Northern Patagonia, Chile.

Marine genomics·2020
Same author

10 W CEP-stable few-cycle source at 2 µm with 100 kHz repetition rate.

Optics express·2018
Same author

Subcycle quantum electrodynamics.

Nature·2017
Same journal

Mechanistic insights into anti-parkinson effect of baicalein: from neuroinflammation and cell death to neurogenesis and synaptic plasticity.

Molecular biology reports·2026
Same journal

Role of Connexin-43: from cancer initiation to cancer metastasis.

Molecular biology reports·2026
Same journal

Corticosteroids in asthma and COPD: an inflammopharmacological perspective on molecular and genetic determinants.

Molecular biology reports·2026
Same journal

IQGAP2 regulates phagocytic-like activity and PD-L1 expression in glioma through the JAK2/STAT3 axis.

Molecular biology reports·2026
Same journal

Naringenin attenuates amiodarone-induced pulmonary fibrosis in rats via modulation of the SIRT1/NF-κB signalling pathway.

Molecular biology reports·2026
Same journal

Paclitaxel and B7-H6 knockdown inhibit HepG2 hepatocellular carcinoma cell viability and migration, while enhancing apoptosis and cell cycle arrest.

Molecular biology reports·2026
See all related articles

The proteasomal system, including the 20S proteasome and its regulators like the 11S and 19S complexes, plays a crucial role in protein degradation. Gamma-Interferon influences some proteasome subunits, but not the 19S complex composition.

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • The proteasome is a multi-subunit protease complex essential for cellular protein homeostasis.
  • Regulators like the 11S and 19S complexes modulate the 20S proteasome's activity and substrate specificity.
  • Gamma-Interferon is known to induce certain proteasome subunits, but its effect on the 19S regulator is less understood.

Purpose of the Study:

  • To investigate the influence of gamma-Interferon on proteasome subunit composition.
  • To explore the dynamics and subunit organization of the 19S proteasome regulator.
  • To identify novel components associated with the proteasomal system.

Main Methods:

  • Analysis of yeast mutants to study 19S regulator subunits.
  • Cloning, sequencing, and identification of novel proteasome-associated proteins.

Related Experiment Videos

  • Investigating the dynamic associations within the 26S proteasome complex.

    • Main Results:

    • Gamma-Interferon induces specific 20S and 11S proteasome subunits but not 19S subunits.
    • Yeast mutant analysis suggests stringent organization of ATPases within the 26S complex, with non-ATPases potentially shuttling substrates.
    • A novel non-ATPase protein associated with the proteasome was identified, but its function remains unknown.

    Conclusions:

    • The proteasome's subunit composition is differentially regulated by gamma-Interferon.
    • The 19S regulator exhibits dynamic associations, with distinct roles for ATPase and non-ATPase subunits.
    • Further research is needed to elucidate the function of newly identified proteasome-associated proteins and viral protein interactions.