Related Experiment Videos
Interferon-gamma production by the human preimplantation embryo
M H Ozornek1, P Bielfeld, J S Krüssel
1Department of OB/GYN, Heinrich Heine University, Duesseldorf, Germany.
Problem:
This study demonstrated that the human embryo produces interferon-gamma (IFNgamma). It is important to know whether IFNgamma can be produced before implantation. Therefore the aim of this study was to evaluate the profile of IFNgamma production between days 2 and 5 after in vitro fertilization.
Method:
Twenty embryos were cultured from day 2 to 5 after fertilization. The embryo stages were checked each day and the media refreshed. IFNgamma levels were estimated by an enzyme-linked immunosorbent assay.
Results:
All embryos produced measurable IFNgamma at least for 1 day. Yields of IFNgamma were: 0.46 +/- 0.45 (n = 4) on day 2, 0.69 +/- 0.52 (n = 19) on day 3, 0.73 +/- 0.52 (n = 15) on day 4, 0.55 +/- 0.32 (n = 11) IU/ml on day 5, respectively. There was no significant difference in the IFNgamma production between in vitro culture days or between the developmental stages of embryos.
Conclusion:
IFNgamma is produced by all the embryos and seems to peak between days 3 and 4, which is just before implantation.