Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genomics via optical mapping. II: Ordered restriction maps

T S Anantharaman1, B Mishra, D C Schwartz

  • 1Computer Science and Chemistry Department, New York University, New York 10012, USA.

Journal of Computational Biology : a Journal of Computational Molecular Cell Biology
|July 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial Expression of Concern: Disruption of transforming growth factor-β signaling through β-spectrin ELF leads to hepatocellular cancer through cyclin D1 activation.

Oncogene·2026
Same author

Editorial Expression of Concern: RING finger-dependent ubiquitination by PRAJA is dependent on TGF-ß and potentially defines the functional status of the tumor suppressor ELF.

Oncogene·2025
Same author

From spectrum to yield: advances in crop photosynthesis with hyperspectral imaging.

Photosynthetica·2025
Same author

Abdominal wall reconstruction (AWR) for post-trauma laparotomy ventral hernia and follow-up assessment of functional quality of life (QOL): experience of a level-1 trauma centre in India.

Hernia : the journal of hernias and abdominal wall surgery·2024
Same author

TB treatment regimen for children: is a 4-month regimen really better than the 6-month regimen?

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2022
Same author

Temporal dysregulation of genes in lamb testis cell during sheeppox virus infection.

Letters in applied microbiology·2022

This study presents an algorithmic approach for creating accurate DNA restriction maps from individual molecule images. The developed software enables rapid, scalable, and high-resolution map construction for various DNA clones.

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Ordered restriction mapping is crucial for DNA analysis.
  • Current methods may lack speed, accuracy, or scalability.
  • Automated DNA mapping requires robust algorithmic solutions.

Purpose of the Study:

  • To develop advanced algorithms for constructing ordered restriction maps.
  • To create high-resolution and high-accuracy DNA maps efficiently.
  • To enhance the scalability of DNA mapping processes.

Main Methods:

  • Utilizing image data from populations of individual DNA molecules (clones).
  • Employing restriction enzyme digestion to generate DNA fragments.
  • Developing and applying novel map-making algorithms.

Related Experiment Videos

Main Results:

  • Successfully constructed ordered restriction maps from clone data.
  • Achieved high resolution and accuracy in the generated maps.
  • Demonstrated the software's routine use in mapping cosmid, lambda, and BAC clones.

Conclusions:

  • The algorithmic approach provides a promising solution for DNA restriction mapping.
  • The developed software is a valuable component of optical mapping automation.
  • The methods offer rapid, scalable, and accurate map construction.