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GAP1 activity is dependent on cAMP in Saccharomyces cerevisiae
A A Amitrano1, D A Saenz, E H Ramos
1Departamento de Bioquímica, Facultad de Medicina, Universidad de Buenos Aires-CONICET, Argentina.
FEMS Microbiology Letters
|June 15, 1997
Abstract:
General amino acid permease (GAP1) activity was evaluated in adenylate cyclase-deficient Saccharomyces cerevisiae to determine the effect of cAMP levels on GAP1 activity. Lowering cAMP concentrations in the culture media led to a decrease in the initial rates of L-citrulline uptake. Kinetics of the amino acid transport system showed a partial loss of transport capacity, with no apparent modifications in permease affinity.