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Evidence for regulation of the NADH peroxidase gene (npr) from Enterococcus faecalis by OxyR
1Department of Biochemistry, Wake Forest University Medical Center, Winston Salem, NC 27157, USA. pross@dpc.teagasc.ie
FEMS Microbiology Letters
|June 15, 1997
Abstract:
We report that the purified Escherichia coli OxyR protein can bind specifically upstream of the gene encoding NADH peroxidase (npr) from Enterococcus faecalis 10C1, to a site located some 144 bp from the promoter. A 34 kDa protein has been identified in crude extracts of E. faecalis that cross-reacts with polyclonal antisera to purified OxyR from E. coli and a protein(s) present in these extracts retards npr DNA fragments in gel shift assays. Taken together with the results of sequence analyses, these observations suggest that enterococcal npr is regulated by OxyR.