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Identification and characterization of thymidylate synthase from Neisseria gonorrhoeae
1Department of Health and Human Services, National Institutes of Health, National Institute of Allergy and Infectious Diseases, USA. jcarlson@pop.niaid.nih.gov
FEMS Microbiology Letters
|June 15, 1997
Abstract:
Thymidylate synthase converts deoxyuridylate to deoxythymidylate. The thyA gene has been cloned and sequenced from Neisseria gonorrhoeae MS11. This gene has an open reading frame of 795-801 bp and encodes a product which shares 71% identity with its Escherichia coli homolog. Unlike its E. coli counterpart, gonococcal thyA has a large, upstream transcribed region (300+ bp) that lacks a translatable reading frame. Gonococcal thyA is capable of complementing an E. coli thyA null mutant and shares similar levels of sensitivity with trimethoprim.