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From gene to screen with yeast

S G Oliver1

  • 1Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology (UMIST), UK. steve.oliver@umist.ac.uk

Current Opinion in Genetics & Development
|June 1, 1997
PubMed
Summary
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The yeast genome project now focuses on gene function using new genome-wide techniques. These methods analyze gene deletions, growth rates, and protein expression for potential human medicine discovery.

Area of Science:

  • * Genomics and molecular biology.
  • * Systems biology and bioinformatics.
  • * Drug discovery and pharmaceutical research.

Background:

  • * The yeast genome sequencing is complete, initiating a new phase focused on gene function.
  • * Advanced techniques are crucial for comprehensive genome-wide functional analysis.

Purpose of the Study:

  • * To detail novel genome-wide functional analysis techniques in yeast.
  • * To explore yeast's potential in identifying human gene functions and drug targets.

Main Methods:

  • * Genome-wide gene deletion with oligonucleotide tagging.
  • * Physiological effect quantification via growth rate and metabolite profiling.
  • * Transcriptional analysis using hybridization arrays.
  • * Proteomic analysis via mass spectrometry and 2D gel electrophoresis.

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Main Results:

  • * Development of efficient methods for gene deletion and functional analysis.
  • * Establishment of techniques for comprehensive transcript and protein profiling.
  • * Identification of strategies for studying gene function across various conditions.

Conclusions:

  • * Yeast serves as a powerful model for understanding gene function.
  • * Functional homologies between yeast and human genes aid in discovering new medicines.
  • * Yeast can be utilized to express human genes for identifying drug targets.