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In vivo alkylation studies with dichlorvos at practical use concentrations
Chemico-Biological Interactions
|October 1, 1977
Summary
Dichlorvos exposure did not methylate rat DNA or RNA. Even at high concentrations, this pesticide is unlikely to damage nucleic acids in mammals when inhaled.
Area of Science:
- Toxicology
- Molecular Biology
- Environmental Health
Background:
- Dichlorvos is an organophosphate insecticide.
- Concerns exist regarding its potential genotoxicity and DNA methylation effects.
Purpose of the Study:
- To investigate whether dichlorvos exposure leads to DNA and RNA methylation in mammalian tissues.
- To assess the risk of nucleic acid damage from inhaled dichlorvos.
Main Methods:
- Male rats were exposed to radiolabeled dichlorvos via inhalation for 12 hours.
- DNA and RNA were isolated from soft tissues and analyzed for methylation at the N7 atom of guanine.
Main Results:
- No detectable methylation of DNA or RNA was observed.
- The detection limits for methylation were extremely low (1 per 6.0 x 10^11 DNA units, 1 per 2 x 10^9 RNA units).
- Less than 0.000001% of the dose would be required for detectable methylation.
Conclusions:
- Dichlorvos does not methylate mammalian nucleic acids under the tested conditions.
- Rapid metabolism and short half-life of methylated guanine prevent accumulation.
- Inhaled dichlorvos at practical concentrations poses no significant risk of nucleic acid methylation.