Related Experiment Video

Updated: Aug 9, 2026

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
11:19

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 25, 2011

[Interaction of ribosomes with membranes in vitro]

T A Borisova, I O Trikash, V K Lishko

    Ukrainskii Biokhimicheskii Zhurnal (1978)
    |September 1, 1996
    PubMed

    Insights

    This study used a fluorescent probe to show that ribosomes directly interact with cell membranes. Ribosome-membrane interactions were more efficient with negatively charged membranes.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Biophysics

    Context:

    • Investigating the mechanisms of protein synthesis and cellular organization.
    • Understanding the role of membrane-ribosome interactions in cellular processes.

    Purpose:

    • To investigate the direct interaction between ribosomes and biological membranes in vitro.
    • To characterize the nature and efficiency of these interactions using a fluorescent probe.

    Summary:

    • A fluorescent probe (R18) was incorporated into ribosomes and membranes to study their interactions.
    • Increased fluorescence indicated ribosome-membrane interaction, with higher efficiency observed for negatively charged liposomes.
    • Ribosomes demonstrated interaction with phospholipid membranes even after protein degradation, suggesting direct lipid-based binding.

    Impact:

    • Provides evidence for direct ribosome-membrane interactions, crucial for understanding protein localization and cellular function.
    • Highlights the influence of membrane charge on ribosome binding.
    • Suggests potential roles for membrane-associated protein synthesis in cellular processes.

    More Related Videos

    Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling06:58

    Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

    Published on: October 7, 2021

    In Vitro Reassociation Assay to Measure the Formation of 80S Ribosomal Particles Using Salt-washed Ribosomal Subunits06:09

    In Vitro Reassociation Assay to Measure the Formation of 80S Ribosomal Particles Using Salt-washed Ribosomal Subunits

    Published on: December 16, 2025

    Related Concept Videos

    Ribosomes01:27

    Ribosomes

    Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
    Ribosomal RNA Synthesis02:53

    Ribosomal RNA Synthesis

    Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
    Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
    Ribosomes01:27

    Ribosomes

    Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
    The Endoplasmic Reticulum01:43

    The Endoplasmic Reticulum

    The endoplasmic reticulum or ER makes up for more than half of the membranes in a cell and accounts for 10% of total cell volume. It is also the primary protein and lipid synthesis factory for most cell organelles, such as the Golgi apparatus, lysosomes, secretory vesicles, and the plasma membrane. Despite being the most extensive and functionally complex subcellular organelle, ER was the last to be discovered. After years of deliberation, Keith Porter and George Palade in the year 1954,...
    Ribosomes01:27

    Ribosomes

    Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
    Ribosome Structure and Assembly
    Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
    Ribosomes01:27

    Ribosomes

    Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
    Ribosome Structure and Assembly
    Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...