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Updated: Jul 26, 2026
Experimental Human Pneumococcal Carriage
Published on: February 15, 2013
Evaluation of transport media for Pasteurella multocida isolates from rabbit nasal specimens
E Kawamoto1, T Sawada, T Maruyama
1Animal Research Center, Tokyo Medical College, Shinjuku, Japan. kawamoto@tokyo-med.ac.jp
Abstract:
A suitable medium for the transport of Pasteurella multocida in nasal specimens from rabbits was investigated by using pure cultures of the organism and nasal swabs from infected rabbits. First, the ability of eight transport media to preserve the viabilities of P. multocida strains isolated from rabbits was studied. Cary-Blair medium and Leibovitz medium no. 15 (L-15) were found to be superior to the other six media tested, enabling survival of the organism for more than 14 days at room temperature. Second, the survival of P. multocida in nasal specimens was evaluated on both Cary-Blair medium and L-15. The recovery rate of the organism from these two media was more than 80 to 90% during 4 days of storage and decreased gradually with increasing preservation time. There were no significant differences (P > 0.05) in recovery rates of the organism between Cary-Blair medium and L-15. On the basis of these results, we recommend the use of Cary-Blair medium for the transport of P. multocida in rabbit nasal specimens because of the ease of transport of nasal swabs by mail.
Insights
Cary-Blair medium and Leibovitz medium no. 15 (L-15) effectively preserve Pasteurella multocida viability in rabbit nasal swabs for over 14 days. Cary-Blair medium is recommended for its ease of transport.
Area of Science:
- Veterinary Microbiology
- Bacteriology
- Animal Health
Background:
- Pasteurella multocida is a significant pathogen in rabbits, causing respiratory infections.
- Effective transport of nasal specimens is crucial for accurate diagnosis and disease management.
- Current transport methods may impact the viability of P. multocida.
Purpose of the Study:
- To evaluate the suitability of different transport media for Pasteurella multocida from rabbit nasal specimens.
- To determine the optimal medium for preserving P. multocida viability during transport and storage.
Main Methods:
- Tested eight transport media for their ability to preserve P. multocida viability using pure cultures.
- Assessed P. multocida survival in rabbit nasal swabs stored in the two superior media (Cary-Blair and L-15).
- Evaluated recovery rates over time at room temperature.
Main Results:
- Cary-Blair medium and Leibovitz medium no. 15 (L-15) supported P. multocida survival for over 14 days at room temperature.
- Both media maintained high recovery rates (80-90%) for P. multocida from nasal specimens for up to 4 days.
- No significant difference in recovery rates was observed between Cary-Blair and L-15.
Conclusions:
- Cary-Blair medium and L-15 are suitable for transporting P. multocida from rabbit nasal specimens.
- Cary-Blair medium is recommended due to its practicality for postal transport of nasal swabs.
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