On the mechanism of hormone recognition and binding by the CCK-B/gastrin receptor

Lipidation with long-chain di-fattyacyl-glycerol moieties was used to anchor gastrin in CCK peptides irreversibly to lipid bilayers lipopeptide transfer to model phospholipid bilayers is fast and quantitative, leading to a different mode of insertion of lipo-gastrin and lipo-CCK in lipid layers. Lipo-gastrin remains exposed to the bulk solvent in a predominantly random coil structure as a consequence of electrostatic repulsion, whereas lipo-CCK exhibits a pronounced tendency to form peptide domains with insertion of its C-terminus into more hydrophobic compartments of the bilayers. Thereby Ca2+ at physiological concentrations favours this aggregational phenomenon. Since both lipo-peptides were found to retain almost full receptor affinity despite their irreversible anchorage to the bilayer, a membrane-bound pathway in the receptor recognition and binding process is indeed possible. According to the data collected in this study, CCK might possibly use this pathway, whereas accumulation of gastrin on the cell membrane with prefolding of the ligand at the water/lipid interface is hardly conceivable. Nevertheless the observed receptor interaction of the deliberately membrane-anchored gastrin offers interesting constraints for computational docking experiments on a modelled CCK-B/gastrin receptor by additionally taking into account information derived from mutagenesis studies. Despite the limitations of such modelling experiments, the resulting picture of the gastrin/receptor complex allowed the visualization and rationalization of the experimental results of the extensive structure-function studies performed previously on this family of gastrointestinal hormones.