Related Experiment Videos
Small peptide libraries: combinatorial split-mix synthesis followed by combinatorial amino acid analysis of selected
H Weinberger1, E Lichte, C Griesinger
1Institute of Organic Chemistry, J.W. Goethe-University of Frankfurt, Frankfurt/Main, Germany.
Abstract:
Peptides from small combinatorial libraries, covalently attached to polymeric TentaGel beads, can be directly sequenced using amino acid analysis. For libraries with restricted diversity, generated by the split-mix synthesis method, the amino acids on a selected single bead identified by pre-column derivatization with o-phthaldialdehyde (OPA) correlate directly with the sequence of a given peptide. This is shown on a tripeptide (343 different compounds) and a tetrapeptide (4096 different compounds) library. This method allows for rapid peptide sequence determination without relying on complex encoding strategies.