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Updated: Jul 29, 2026
Procedure for Human Saphenous Veins Ex Vivo Perfusion and External Reinforcement
Published on: October 1, 2014
Influence of twist on vein and PTFE graft hemodynamics: an ex vivo experimental study
S Karacagil1, S Bowald, B Almgren
1Department of Surgery, University Hospital, Uppsala, Sweden.
Objective:
In an ex vivo infusion model, the effect of different factors affecting the critical level of twist in vein and PTFE grafts was analysed.
Setting:
University Hospital, Department of Surgery.
Experimental Design:
Saline perfusion of grafts with various diameter and length under constant high (90 ml/min) and low (50 ml/min) flow rates against various peripheral resistance was performed.
Measures:
The pressure changes in the grafts were measured at increasing increments of twisting.
Results:
The critical level of twist appears to be dependent on the diameter, length, stretching, status of peripheral resistance, amount of volume flow and the type of graft material, varying between 90 to more than 360 degrees. In vein grafts critical twisting appeared earlier with a decrease in diameter, shortening in length, high peripheral resistance, high flow rate and stretching. PTFE grafts especially with external spiral support were resistant to twist.
Conclusions:
These data suggest that the critical level of graft twist is dependent upon multiple factors and moderate or even high grade twist with especially externally supported PTFE grafts regardless of length and long mobilized vein grafts do not lead to hemodynamic changes. On the other hand total cessation of flow occurs at lower degrees of twist in stretched, short vein grafts.
