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Matrix synthesis and cell proliferation in repaired flexor tendons within e-PTFE reconstructed flexor tendon sheaths
1Department of Hand Surgery, University Hospital, Malmö, Sweden.
Journal of Hand Surgery (Edinburgh, Scotland)
|October 1, 1996
Summary
Expanded polytetrafluoroethylene (e-PTFE) membranes may help prevent adhesions after flexor tendon surgery. While e-PTFE did not significantly impact cell proliferation in healing tendons, it slightly reduced protein synthesis.
Area of Science:
- Biomaterials Science
- Orthopedic Surgery
- Regenerative Medicine
Background:
- Adhesions following flexor tendon surgery can restrict motion.
- Expanded polytetrafluoroethylene (e-PTFE) is a potential barrier material to prevent adhesions.
Purpose of the Study:
- To evaluate the effect of e-PTFE membranes on the cellular activity of healing tendons.
- To compare matrix component synthesis and DNA synthesis in tendons covered with e-PTFE versus sham controls.
Main Methods:
- Thirty rabbits underwent divided and sutured flexor tendon repair.
- Tendons were covered with e-PTFE membranes or sham controls.
- In vitro culture with radiolabeled precursors (35S-sulphate, 3H-proline, 3H-thymidine) assessed synthesis at 12 weeks.
Main Results:
- No adverse tissue reactions or tendon disruptions were observed.
- Synthesis of matrix components and DNA were similar between e-PTFE and sham groups.
- Synthesis of non-collagen protein and total protein/collagen content were lower in the e-PTFE group.
Conclusions:
- e-PTFE membranes may not significantly impair cell proliferation in healing tendons.
- e-PTFE use may lead to a moderate reduction in protein synthesis in healing tendons.
- e-PTFE shows potential as an adhesion barrier without major detrimental effects on tendon cellularity.