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Mouse CD1 is mainly expressed on hemopoietic-derived cells
L Brossay1, D Jullien, S Cardell
1Department of Microbiology and Immunology, University of California, Los Angeles 90095, USA.
Journal of Immunology (Baltimore, Md. : 1950)
|August 1, 1997
Summary
Mouse CD1 (mCD1) is primarily expressed on immune cells, not intestinal cells as previously thought. Its distribution aligns with NK1.1+ T cells, suggesting a key antigen-presenting role.
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Background:
- Mouse CD1 (mCD1) is a class I-like molecule outside the MHC.
- mCD1 presents hydrophobic peptides to CD8+ T cells and is recognized by IL-4 secreting NK1.1+ T cells.
- Previous reports indicated mCD1 expression on intestinal epithelial cells, contrasting with NK1.1+ T cell distribution.
Purpose of the Study:
- To investigate the tissue distribution of mouse CD1 (mCD1).
- To clarify the expression pattern of mCD1 in relation to NK1.1+ T cells.
- To explore potential beta2m-independent mCD1 expression.
Main Methods:
- Generation of new monoclonal antibodies (mAbs) against mCD1.
- Analysis of mCD1 tissue distribution in normal and beta2m-deficient mice.
- Utilizing mCD1 autoreactive T hybridomas for recognition assays.
Main Results:
- mCD1 is predominantly expressed on hematopoietic cells (T cells, B cells, macrophages, dendritic cells).
- mCD1 is largely absent from intestinal epithelial cells.
- B cells from beta2m-deficient mice showed recognition by mCD1 hybridomas, suggesting alternative forms or related molecules.
Conclusions:
- The primary expression of mCD1 on immune cells correlates with NK1.1+ T cell distribution.
- This expression pattern supports a significant antigen-presenting function for mCD1.
- Evidence suggests potential beta2m-independent mCD1 expression or related molecules.