Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mutational analysis of acute-phase response factor/Stat3 activation and dimerization

J Sasse1, U Hemmann, C Schwartz

  • 1Institute of Biochemistry, Rheinisch-Westfälische Technische Hochschule Aachen, Germany.

Molecular and Cellular Biology
|August 1, 1997
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unified Gait Event Detection using Temporal Convolutional Network and Bayesian Optimization.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same author

A new field-test for assessing the medial and lateral hamstring strength at long-muscle length.

Journal of bodywork and movement therapies·2024
Same author

Answer to: Letter received concerning the "EAPM position statement: Use of appropriate terminology for situations related to inadequate fetal oxygenation in labor" from Savshenko J and Wendel SB: "On importance of consistent terminology and mutual understanding".

European journal of obstetrics, gynecology, and reproductive biology·2024
Same author

Posterior thigh muscles activity during the active H-test: An electromyographic and kinematic analysis.

Journal of bodywork and movement therapies·2024
Same author

Highly efficient and sensitive membrane-based concentration process allows quantification, surveillance, and sequencing of viruses in large volumes of wastewater.

Water research·2023
Same author

Long-term follow-up of patients with extensive segmental infantile hemangioma of the cervical or facial region: A French single-center prospective study.

Archives de pediatrie : organe officiel de la Societe francaise de pediatrie·2023

Truncating the carboxy-terminal region of signal transducer and transcription (STAT) factors like Stat3 can lead to constitutive activation. However, the ability of these truncated Stat3 proteins to regulate gene transcription varies by cell type.

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Transcription Factors

Background:

  • Signal transducer and transcription (STAT) factors regulate gene expression in response to extracellular signals.
  • Activation of STAT factors involves tyrosine phosphorylation, dimerization, and nuclear translocation.

Purpose of the Study:

  • To investigate the functional role of the carboxy-terminal region of Stat3 in its activation, dimerization, and transactivation.
  • To analyze how truncations in Stat3 affect its signaling capabilities.

Main Methods:

  • Utilized deletion and point mutants of Stat3 in COS-7 and HepG2 cells.
  • Employed reporter gene assays to assess transactivation potential.
  • Analyzed Stat3 dimerization and activation mechanisms.

Related Experiment Videos

Main Results:

  • Truncation of 55 carboxy-terminal amino acids resulted in constitutive Stat3 activation in COS-7 cells.
  • Mutations affecting residues near and far from Y705 blocked Stat3 dimerization.
  • Carboxy-terminally truncated Stat3 failed to transactivate an IL-6-responsive promoter in COS-7 cells but functioned similarly to full-length Stat3 in HepG2 cells.

Conclusions:

  • Both carboxy- and amino-terminal regions of Stat3 are crucial for its activation and dimerization.
  • Cell-type-specific mechanisms dictate whether Stat3 requires its carboxy-terminal transactivation domain for target gene regulation.