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Published on: July 11, 2014
Assay for valproic acid and its E-delta2 metabolite in rat plasma by capillary gas chromatography without prior
1Biopharmaceutics Research Branch, Center for Drug Evaluation and Research, Food and Drug Administration, Rockville, MD 20850, USA.
Abstract:
A new and improved gas chromatographic assay method for valproic acid and a metabolite, E-delta2 valproic acid, in rat plasma has been developed. The assay has sufficient sensitivity to measure free levels of the parent drug and metabolite. By employing a Stabilwax-DA capillary column, symmetrical chromatographic peaks were obtained without the need for prior derivatization. Standard curves for valproic acid were linear from 0.1 to 640 microg/ml. Standard curves for the metabolite were linear from 0.1 to 556 microg/ml.
