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Updated: Aug 6, 2026

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library
Published on: June 20, 2014
A single-bead decode strategy using electrospray ionization mass spectrometry and a new photolabile linker:
B B Brown1, D S Wagner, H M Geysen
1Diversity Sciences Department, Glaxo Wellcome Inc., Research Triangle Park, NC 27709, USA.
Abstract:
A new linker that employs a photosensitive 3-amino-3-(2-nitrophenyl)propionyl functionality (ANP-resin) has been developed for the preparation of C-terminal carboxamides. A wide range of carboxamides were prepared and identified using the ANP-resin and electrospray ionization mass spectrometry. A single bead containing tripeptide Fmoc-Asp-Arg(Tos)-Val-NH2 was isolated, photocleaved and the peptide was characterized by tandem mass spectrometry, thereby verifying a library decode strategy that avoids complex tagging procedures.
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