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Related Experiment Videos

Cellular electrophoretic mobility data: a first approach to a database

G G Slivinsky1, W C Hymer, J Bauer

  • 1Institute of Zoology, Academy of Sciences, Alma Ata, Kazachsthan.

Electrophoresis
|June 1, 1997
PubMed
Summary
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This study compiled 40 years of eukaryotic cell electrophoretic mobility data, revealing most cells share similar mobilities. This finding aids cell purification and highlights strong biological control over cell electrophoresis.

Area of Science:

  • Cell Biology
  • Biophysics

Background:

  • Electrophoretic mobility is a key biophysical property of cells.
  • Understanding cellular electrophoretic mobility aids in cell separation and characterization.

Purpose of the Study:

  • To compile and analyze a comprehensive dataset of eukaryotic cellular electrophoretic mobility.
  • To assess the variability and commonalities in electrophoretic mobility across diverse eukaryotic cell types.

Main Methods:

  • Literature review and data compilation of cellular electrophoretic mobility values from worldwide publications over 40 years.
  • Analysis of 288 types of eukaryotic cells, including historical data not readily available through modern retrieval systems.

Main Results:

  • A comprehensive list of cellular electrophoretic mobility values for 288 eukaryotic cell types was created.

Related Experiment Videos

  • Most eukaryotic cells exhibit electrophoretic mobilities within a narrow range, 40% above to 50% below that of human erythrocytes.
  • The compiled data includes both recent and historical findings, some of which are difficult to access via current search methods.

    • Conclusions:

    • The study provides a valuable resource for scientists involved in cell population purification.
    • The observed similarity in electrophoretic mobilities across many eukaryotic cells supports the hypothesis of strong biological regulation.