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Updated: Aug 7, 2026
Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells
Published on: December 18, 2010
Colony stimulating factor-1 in human follicular fluid
1Department of Obstetrics and Gynecology, Albert Einstein College of Medicine, Bronx, New York 10461, USA. BWITT@AECOM.YU.EDU
Objective:
To evaluate colony stimulating factor-1 (CSF-1) concentrations in serum and follicular fluid (FF) at the time of oocyte retrieval and to test for presence of messenger RNA (mRNA) for CSF-1 and its receptor, c-fms, in FF cells.
Design:
Collection of serum and FF at the time of oocyte retrieval.
Setting:
A university IVF program.
Patient(S):
Forty-five women undergoing oocyte retrieval for IVF.
Intervention(S):
Serum and FF were obtained from 24 women, and FF only was obtained from 21 women.
Main Outcome Measure(S):
Colony-stimulating factor-1 concentrations were determined by RIA, and the presence of mRNA for CSF-1 and c-fms was determined by reverse transcriptase-polymerase chain reaction.
Result(S):
Mean FF concentrations of CSF-1 were significantly higher than mean serum levels (10.0 +/- 1.3 and 3.6 +/- 0.3 (+/-SE) ng/mL, respectively). Colony-stimulating factor-1 and c-fms message were detected in FF cells, and alternatively spliced forms of CSF-1 message were present.
Conclusion(S):
The presence of CSF-1, a primary regulator of tissue macrophages, in FF, and the presence of mRNA for CSF-1 and its receptor c-fms in FF-derived cells, suggest a role for this growth factor in ovarian function.
