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Tissue factor expression during monocyte-macrophage differentiation

M M van den Eijnden1, S I Steenhauer, P H Reitsma

  • 1Dept. of Hematology, University Hospital, Leiden, The Netherlands.

Thrombosis and Haemostasis
|June 1, 1997
PubMed
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During monocyte differentiation into macrophages, tissue factor (TF) is transiently expressed. Macrophages maintain responsiveness to lipopolysaccharide (LPS) for TF induction, even with spontaneous TF expression.

Area of Science:

  • Immunology
  • Hematology
  • Cell Biology

Background:

  • Tissue factor (TF) is a key procoagulant in monocytes and macrophages.
  • Understanding TF expression during monocyte differentiation is crucial for hemostasis research.

Purpose of the Study:

  • To investigate the impact of long-term culture (differentiation) on spontaneous and LPS-induced TF expression in human monocytes.
  • To analyze TF expression at mRNA, antigen, and functional activity levels.

Main Methods:

  • Isolated human monocytes were cultured long-term on Teflon membranes (Mo-S) and plastic dishes (Mo-A).
  • TF expression (mRNA, antigen, VIIa-cofactor activity) was assessed.
  • Macrophage-specific markers (CD71, mannose receptor, scavenger receptor) were monitored.
  • Cells were stimulated with bacterial lipopolysaccharide (LPS).

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Main Results:

  • Monocytes exhibited transient "spontaneous" TF expression during differentiation, peaking between days 3-5.
  • This spontaneous expression correlated with increased macrophage markers.
  • LPS induced additional TF mRNA, antigen, and activity in both Mo-S and Mo-A.
  • TF responsiveness to LPS was reduced during the peak spontaneous TF expression period (days 3-5).

Conclusions:

  • Monocyte-to-macrophage transition involves transient, spontaneous TF expression.
  • Human monocytes maintain their responsiveness to LPS for TF induction throughout differentiation.