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Related Experiment Videos

Clathrin-coated vesicle formation and protein sorting: an integrated process

S L Schmid1

  • 1Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037, USA.

Annual Review of Biochemistry
|January 1, 1997
PubMed
Summary

Clathrin-coated vesicles mediate essential cell transport. Their coat proteins directly aid cargo sorting and packaging, integrating vesicle budding with protein sorting for efficiency.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Clathrin-coated vesicles (CCVs) are the most studied transport vesicles.
  • CCVs are crucial for endocytosis and lysosomal hydrolase transport from the trans-Golgi network.

Purpose of the Study:

  • To elucidate the functional and structural roles of clathrin and adaptor protein complexes in CCV formation.
  • To understand the interplay between coat constituents, membranes, and cargo sorting signals.

Main Methods:

  • Utilized cell-free assays to study coat assembly.
  • Investigated membrane binding and coated vesicle budding processes.
  • Analyzed interactions between clathrin, adaptor proteins, membranes, and cargo.

Main Results:

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  • Detailed functional and structural insights into CCV machinery were obtained.
  • Demonstrated that coat constituents shape vesicles and directly package cargo.
  • Established a functional integration between protein sorting and vesicle budding.

Conclusions:

  • The interplay between CCV machinery and cargo ensures sorting fidelity and packaging efficiency.
  • This integration allows for the modulation of vesicular trafficking based on cellular demand.