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Updated: Oct 21, 2026

The Successive Alleys Test of Anxiety in Mice and Rats
Published on: June 17, 2013
Role of different neurotransmitter systems in the cholecystokinin octapeptide-induced anxiogenic response in rats
1Institute of Pathophysiology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Abstract:
The possible involvement of different neurotransmitter systems in the anxiogenic action of cholecystokinin octapeptide sulphate ester (CCK-8) was investigated in rats. Intracerebroventricularly (i.c.v.) administered CCK-8 induced an anxiogenic response in an elevated plus-maze test. Pretreatment with dopaminergic, muscarinergic acetylcholine receptor blockers and an opiate receptor antagonist blocked the anxiogenic response to CCK-8. The alpha and beta adrenoreceptor, the GABA receptor and the 5-hydroxytryptamine (5-HT) receptor blockers were not able to modulate the 'anxiogenic-like' effect of CCK-8. The results suggest that the anxiogenic effects of CCK-8 are mediated via different neurotransmitters and the anxiogenic action can be prevented by receptor blockers to these transmitters.
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