The estimation of age of bloodstains by HPLC analysis

A new HPLC system is described for estimating the age of bloodstains deposited on clothing. In addition to the decomposition peak designated as "X" and reported earlier in the literature, several other ageing processes were studied and found suitable for estimation of age of the stains. The various processes can be used independently of each other for estimation of bloodstain age when the storing temperature is known. Moreover, the ratio between the different peaks formed by ageing is practically independent of temperature between 0 degree C and 37 degrees C.