Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Validation studies of the CTT STR multiplex system

B Budowle1, T R Moretti, K M Keys

  • 1Forensic Science Research and Training Center, FBI Academy, Quantico, Virginia 22135, USA.

Journal of Forensic Sciences
|July 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

4th DNA Forensic Symposium: Challenges and future directions in Africa.

Forensic science international. Synergy·2024
Same author

Association of whole mtDNA, an NADPH G11914A variant, and haplogroups with high physical performance in an elite military troop.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2021
Same author

Evaluation of InnoTyper® 21 in a sample of Rio de Janeiro population as an alternative forensic panel.

International journal of legal medicine·2017
Same author

Letter to the editor: Are the doors opened to a genetic-based algorithm for personalized resistance training?

Biology of sport·2017
Same author

Global genetic variation of select opiate metabolism genes in self-reported healthy individuals.

The pharmacogenomics journal·2017
Same author

D5S2500 is an ambiguously characterized STR: Identification and description of forensic microsatellites in the genomics age.

Forensic science international. Genetics·2016

This study validates multiplex amplification of three short tandem repeat (STR) loci (CSF1PO, TPOX, THO1) for forensic use. The system reliably analyzes DNA from various challenging forensic samples, confirming its applicability in casework.

Area of Science:

  • Forensic Science
  • Molecular Biology
  • Genetics

Background:

  • Short tandem repeat (STR) analysis is a cornerstone of forensic DNA profiling.
  • Multiplex amplification of multiple STR loci enhances discrimination power and efficiency in forensic investigations.
  • Standardization and validation of STR typing systems are crucial for reliable casework application.

Purpose of the Study:

  • To define optimal typing conditions for multiplex amplification of CSF1PO, TPOX, and THO1 STR loci.
  • To evaluate the forensic applicability and robustness of the GenePrint STR System for casework.
  • To assess the impact of various simulated forensic conditions on STR profile integrity.

Main Methods:

  • Utilized the GenePrint STR System (Promega) for multiplex amplification of CSF1PO, TPOX, and THO1.

Related Experiment Videos

  • Analyzed mixed body fluid samples to assess mixture interpretation capabilities.
  • Determined the sensitivity limits of the STR typing system.
  • Evaluated DNA samples from casework and simulated forensic conditions, including chemical contaminants, various substrates, microbial contamination, sunlight, and storage.

    • Main Results:

    • Reliable STR typing results were obtained from biological samples subjected to diverse environmental insults.
    • The GenePrint STR System demonstrated consistent performance across various simulated forensic conditions.
    • STR profiles remained largely intact and interpretable despite exposure to contaminants, substrates, and environmental factors.

    Conclusions:

    • The multiplex amplification of CSF1PO, TPOX, and THO1 STR loci using the GenePrint STR System is suitable for forensic applications.
    • The system exhibits robustness and reliability when analyzing DNA from challenging forensic samples.
    • This validated method enhances the capabilities of forensic casework analysis.