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Blockage of mesenteric lymphatic flow in rats
H Tsuchiya1, K Nagashima, T Nemoto
1Department of Pediatric Surgery, Koshigaya Hospital, Dokkyo University School of Medicine, 2-1-50 Minami-Koshigaya, Koshigaya, Saitama 343, Japan.
Pediatric Surgery International
|July 1, 1997
Summary
Blocking mesenteric lymphatic flow (MLF) in rats caused abnormal weight gain and fluid buildup. Lymphatic vessels dilated, leading to leakage and chylous ascites, with lymph node changes observed.
Area of Science:
- Vascular Biology
- Lymphatic System Research
- Experimental Pathology
Background:
- The mesenteric lymphatic system is crucial for fluid balance and nutrient absorption.
- Understanding the consequences of mesenteric lymphatic flow (MLF) blockage is vital for diagnosing and treating related disorders.
Purpose of the Study:
- To establish an experimental model for studying mesenteric lymphatic flow (MLF) blockage in rats.
- To investigate the morphologic changes in mesenteric lymphatic vessels (MLV) and lymph nodes following MLF blockage.
Main Methods:
- An experimental model of MLF blockage was created in rats.
- Mesenteric lymphangiography was performed to visualize MLV changes and contrast medium leakage.
- Histopathologic examination (light and electron microscopy) was used to analyze lymph node morphology.
Main Results:
- Rats with MLF blockage exhibited impaired weight gain.
- Lymphangiography showed MLV dilation, reverse flow, and contrast leakage, causing chylous ascites.
- Histopathology revealed dilated lymphatic sinuses and altered lymphocytes in mesenteric lymph nodes due to lymph stagnation.
Conclusions:
- The experimental model effectively demonstrated the pathological effects of MLF blockage.
- MLF blockage leads to significant morphologic alterations in MLV and lymph nodes, resulting in chylous ascites.