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Fibrin induction of tissue factor expression in human vascular endothelial cells
J Contrino1, S Goralnick, J Qi
1Department of Medicine, University of Connecticut Health Center, Farmington 06030, USA.
Background:
For the present study, we hypothesized that fibrin is an inducer of tissue factor (TF) expression in vascular endothelial cells in vitro and in vivo.
Methods And Results:
To test the in vitro aspect of this hypothesis, human umbilical vein endothelial cells (HUVECs) were cocultured with physiologically relevant concentrations of fibrin (0.03 to 1.0 mg fibrin/mL) for various times (0.5 to 24 hours), and TF expression was compared with that in unstimulated HUVECs (media control). Results demonstrated that fibrin induced a time- and dose-dependent increase in TF antigen expression, functional TF procoagulant activity, and TF mRNA in HUVECs.
Conclusions:
These studies demonstrate that fibrin can directly regulate TF expression in HUVECs in vitro.