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High-resolution recombinational map of mouse chromosome 16
R H Reeves1, E E Rue, M P Citron
1Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA. rreeves@welchlink.welch.jhu.edu
Genomics
|July 15, 1997
Summary
Researchers created a detailed genetic map of mouse Chromosome 16 using six crosses. This map aids in physical mapping and understanding chromosome recombination.
Area of Science:
- Genetics
- Genomics
- Mammalian Genetics
Background:
- Mouse Chromosome 16 (Chr 16) is crucial for genetic research.
- Establishing high-resolution genetic maps is essential for understanding chromosome structure and function.
Purpose of the Study:
- To construct a sex-averaged recombinational map of mouse Chr 16.
- To identify recombination sites and order genetic markers for physical map assembly.
Main Methods:
- Utilized five intersubspecific backcrosses and one intercross involving 1155 progeny.
- Employed interval mapping to order 123 markers, including reference markers, genes, and simple sequence length polymorphisms (SSLPs).
Main Results:
- Developed a 56 cM genetic map covering most of mouse Chr 16.
- Identified 425 recombination sites and integrated 123 markers, including 87 SSLPs.
- The map provides a resolution with an average distance of less than 500 kb between crossover sites.
Conclusions:
- The established genetic map serves as a valuable resource for YAC-based physical map assembly of Chr 16.
- The high density of markers and recombination sites facilitates detailed genomic studies.