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Related Experiment Videos

Regional Mapping Strategies Utilizing Microcell Hybrids

Johnson-Pais1, Leach

  • 1Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas, 78284

Methods (San Diego, Calif.)
|February 1, 1996
PubMed
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Microcell hybrids aid human chromosome mapping, but fragmentation can occur. Radiation-reduced hybrids offer a method to map chromosome regions lacking selectable markers, enabling linear chromosome mapping.

Area of Science:

  • Genetics
  • Molecular Biology
  • Genomics

Background:

  • Microcell hybrids are valuable tools for human chromosome mapping.
  • Chromosome transfer can lead to donor chromosome fragmentation, resulting in hybrids with limited retained regions and other fragments.
  • Monochromosomal microcell hybrids serve as donor cell lines for generating radiation-reduced hybrids.

Purpose of the Study:

  • To present a protocol and overview for constructing and analyzing radiation-reduced hybrid panels.
  • To highlight the utility of radiation-reduced hybrids for mapping chromosome regions without selectable markers.
  • To explain how chromosome-specific sequences in both fragment-containing and radiation-reduced hybrids can be used for linear chromosome mapping.

Main Methods:

  • Utilizing microcell-mediated chromosome transfer to create hybrid cell lines.

Related Experiment Videos

  • Generating radiation-reduced hybrids from monochromosomal microcell hybrids.
  • Analyzing chromosome-specific sequences in both fragment-containing and radiation-reduced hybrids.

    • Main Results:

    • Fragment-containing microcell hybrids retain specific chromosome regions and other fragments.
    • Radiation-reduced hybrids allow mapping of chromosome regions lacking selectable markers.
    • The presence or absence of chromosome-specific sequences enables linear chromosome mapping.

    Conclusions:

    • Radiation-reduced hybrids are essential for mapping chromosome regions lacking selectable markers.
    • A comprehensive protocol for radiation-reduced hybrid panel construction and analysis is provided.
    • Both fragment-containing and radiation-reduced hybrids are instrumental in constructing linear chromosome maps.