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DNA sequence encodes information for nucleosome array formation

K Liu1, A Stein

  • 1Department of Biological Sciences, Purdue University, West Lafayette, Indiana, 47907, USA.

Journal of Molecular Biology
|July 25, 1997
PubMed
Summary
This summary is machine-generated.

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DNA base sequence influences how nucleosomes (DNA-protein complexes) arrange into chromatin. This sequence-dependent organization creates a mosaic pattern of ordered and less-ordered chromatin regions.

Area of Science:

  • Molecular Biology
  • Genetics
  • Chromatin Structure

Background:

  • Nucleosomes are fundamental units of DNA packaging in eukaryotes.
  • The arrangement of nucleosomes into higher-order chromatin structures is crucial for genome regulation.
  • The role of DNA sequence in dictating chromatin organization remains an active area of research.

Purpose of the Study:

  • To investigate the impact of specific DNA base sequences on nucleosome array formation.
  • To compare in vitro chromatin assembly with native chromatin organization in vivo.
  • To elucidate the relationship between DNA sequence and the regularity of nucleosome arrays.

Main Methods:

  • Assembly of DNA clones into chromatin using a defined in vitro system.
  • Analysis of nucleosome arrangements in native chromatin within chicken liver nuclei.

Related Experiment Videos

  • Examination of approximately 100 kilobase-pair contiguous DNA regions using cosmid clones.

    • Main Results:

    • Observed sequence-dependent variations in nucleosome array regularity and repeat length, consistent between in vitro and in vivo experiments.
    • Identified a mosaic chromatin organization, with ordered regions ( < 2 kb) alternating with less-ordered regions.
    • Demonstrated that linker histone-dependent alignment, relative to distant positioned nucleosomes, underlies a 210 bp periodicity in ordered arrays.

    Conclusions:

    • DNA base sequence is a primary determinant of chromatin organization, dictating a mosaic structure.
    • Nucleosome array regularity and repeat length are sequence-encoded properties.
    • Linker histones play a role in establishing long-range nucleosome ordering within specific DNA sequences.