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Updated: Oct 1, 2026

Oligopeptide Competition Assay for Phosphorylation Site Determination
Published on: May 18, 2017
Determination of phosphorylation sites in peptides and proteins employing a volatile Edman reagent
W H Fischer1, C A Hoeger, J Meisenhelder
1Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, California 92037, USA. Fischer@sc2.salk.edu
Abstract:
A manual Edman degradation protocol has been developed that allows the identification of phosphorylation sites in 32P-labeled peptides at the subpicomole level. By using both a volatile reagent, trifluoroethyl isothiocyanate, and volatile buffers, extraction steps are rendered unnecessary and cycle times can be reduced to 45 min. The protocol was employed to identify the site of phosphorylation in phosphoserine- and phosphotyrosine-containing peptides.
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