Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Accurate mass measurements using MALDI-TOF with delayed extraction

E J Takach1, W M Hines, D H Patterson

  • 1PerSeptive Biosystems, Inc., Framingham, Massachusetts 01701, USA.

Journal of Protein Chemistry
|July 1, 1997
PubMed
Summary

Enhanced matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry offers unparalleled accuracy for biopolymer analysis. This technique achieves mass measurement accuracy of a few parts per million (ppm), improving peptide identification and elemental composition determination.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A neuroimaging measure to capture heterogeneous patterns of atrophy in Parkinson's disease and dementia with Lewy bodies.

NeuroImage. Clinical·2024
Same author

A true congenital pancreatic cyst in a dog.

BMC veterinary research·2022
Same author

Association between multimorbidity and undiagnosed obstructive sleep apnea severity and their impact on quality of life in men over 40 years old.

Global health, epidemiology and genomics·2018
Same author

Prenatal exposure to vapors of gasoline-ethanol blends causes few cognitive deficits in adult rats.

Neurotoxicology and teratology·2015
Same author

Comparison of insulin lispro protamine suspension versus insulin glargine once daily added to oral antihyperglycaemic medications and exenatide in type 2 diabetes: a prospective randomized open-label trial.

Diabetes, obesity & metabolism·2013
Same author

Photon-induced dissociation with a four-sector tandem mass spectrometer.

Journal of the American Society for Mass Spectrometry·2013

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Mass Spectrometry

Background:

  • Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry is crucial for biopolymer analysis.
  • Existing limitations in mass measurement accuracy and resolution hinder detailed analysis.
  • Advancements are needed to enhance the precision of MALDI-TOF techniques.

Purpose of the Study:

  • To improve mass measurement accuracy and resolution in MALDI-TOF mass spectrometry.
  • To demonstrate the capability of distinguishing amino acids like Lysine (Lys) from Glutamine (Gln) in peptides.
  • To enable accurate elemental composition determination for small molecules.

Main Methods:

  • Implementation of delayed extraction technology.
  • Utilization of a reflecting analyzer in the MALDI-TOF instrument.

Related Experiment Videos

  • Analysis of peptide mixtures and tryptic digests using the enhanced MALDI-TOF system.

    • Main Results:

    • Achieved mass measurement accuracy of a few parts per million (ppm).
    • Demonstrated accuracy below 5 ppm for peptides (900-3700 Da).
    • Obtained an average error less than 3.5 ppm for myoglobin tryptic digest peaks.

    Conclusions:

    • The enhanced MALDI-TOF technique significantly improves mass measurement accuracy.
    • Increased accuracy refines database searching by reducing candidate possibilities.
    • This advancement is vital for precise biopolymer characterization and molecular identification.