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Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
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High-sensitivity mass spectrometry for analysis of posttranslational modifications

T Takao1, T Shimizu, S Ikegami

  • 1Institute for Protein Research, Osaka University, Japan. tak@protein.osaka.u.ac.jp

Journal of Protein Chemistry
|July 1, 1997
PubMed
Summary

Pulsed fast atom bombardment ionization (pulsed-FAB) mass spectrometry enhances tandem mass spectrometry (MS/MS) sensitivity for analyzing minute samples. This technique identified a starfish nuclear protein p28 as a heterodimer of histones H2B and H4, cross-linked at specific residues.

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