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Structure, function and regulation of Ca2+-sensitive cytosolic phospholipase A2 (cPLA2)
1Cardiovascular Research, Lilly Research Laboratories, Indianapolis, IN 46285, USA. Kramer_Ruth_M@Lilly.com
FEBS Letters
|June 23, 1997
Summary
The 85-kDa cytosolic phospholipase A2 (cPLA2) enzyme is activated by calcium and releases fatty acids. These fatty acids are precursors to inflammatory molecules, making cPLA2 a therapeutic target.
Area of Science:
- Biochemistry
- Cell Biology
- Enzymology
Background:
- The 85-kDa cytosolic phospholipase A2 (cPLA2) is a key enzyme involved in cellular signaling pathways.
- Its activation is calcium-dependent and it preferentially hydrolyzes phospholipids containing arachidonic acid.
Purpose of the Study:
- To elucidate the functional properties and catalytic mechanism of cPLA2.
- To understand the role of cPLA2 in cellular processes and disease states.
Main Methods:
- Studies on the activation of cPLA2 by various cellular stimuli.
- Analysis of the products generated by cPLA2 hydrolysis.
Main Results:
- cPLA2 activation occurs at calcium concentrations found in stimulated cells.
- Hydrolysis of phospholipids by cPLA2 liberates fatty acids and lysophospholipids.
- These products serve as precursors for eicosanoids and platelet-activating factor.
Conclusions:
- cPLA2 plays a critical role in the production of signaling molecules involved in inflammation and degeneration.
- The enzyme's unique properties make it a promising target for therapeutic intervention in inflammatory and degenerative diseases.